The key difference between genomic DNA and plasmid DNA isolation is that the genomic DNA isolation targets the extraction of genomic DNA while the plasmid DNA isolation targets the extraction of plasmid DNA of the bacteria.
DNA isolation is a chemical process that used to isolate DNA from different species or from different samples. DNA isolation is important in downstream molecular biology techniques, such as gel electrophoresis, polymerase chain reaction and DNA sequencing techniques. Therefore, isolation of DNA is an essential chemical process in molecular biological studies. Based on the purpose of the research, sometimes it is necessary to isolate genomic DNA. In addition, some studies focus on isolating plasmid DNA from bacteria. Genomic DNA isolation is the process of isolating genomic DNA from a prokaryotic or a eukaryotic sample. The steps of isolation differ according to the type of the cell in which the DNA is isolating. Plasmid DNA isolation is the process of isolating plasmid DNA from a bacterial cell. In comparison to genomic DNA isolation, the overall process is complex in plasmid DNA isolation.
1. Overview and Key Difference
2. What is Genomic DNA Isolation
3. What is Plasmid DNA Isolation
4. Similarities Between Genomic DNA and Plasmid DNA Isolation
5. Side by Side Comparison – Genomic DNA vs Plasmid DNA Isolation in Tabular Form
What is Genomic DNA Isolation?
Genomic DNA isolation is the process of extracting whole genomic DNA of an organism. This particular process involves three main events. They are, cell lysis or nuclear lysis, protein degradation or proteolysis and precipitation of genomic DNA. The lysis step may differ according to the cell type. In prokaryotes, since there is a peptidoglycan cell wall, the first step should be the breakdown of the cell wall. On the other hand, in eukaryotes, the lysis step involves the breakdown of the plasma membrane and the nuclear membrane to take out the DNA to the exterior. In contrast, special steps are essential to lyse the plant and fungal cell walls.