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Difference Between Population Doubling and Passage Number | Population Doubling vs Passage Number


The key difference between population doubling and passage number relies on the role they play in cell culture. Population doubling is the time taken for the cells in a culture medium to double its cell population while passage number refers to the number of times the cell culture has been doubled from its primary culture.

Population doubling and passage number are two important aspects of cell culture. These values help to determine the cell counts and the quality of cell culture. Population doubling is a measurement of time. In contrast, passage number is an integral measurement of the number of times the cells have been subcultured from primary cell culture. These values are very important in determining the quality of the cell culture and for various downstream experimental activities.

CONTENTS

1. Overview and Key Difference
2. What is Population Doubling
3. What is Passage Number
4. Similarities Between Population Doubling and Passage Number
5. Side by Side Comparison – Population Doubling vs Passage Number in Tabular Form
6. Summary

What is Population Doubling?

Population doubling is the time it takes to double the number of cells in a population. Population doubling is a measurement of time; this is often a measure in seconds. In contrast, population doubling level is the number of times population doubling can be achieved. You can determine the population doubling level using the following formula:

Population doubling level = 3.32 (log (total viable cells at harvest / total viable cells at seed))

Difference Between Population Doubling and Passage Number | Population Doubling vs Passage Number